We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis, structural determination, and molecular modeling to enhance binding affinity.

Jan 10, 2023 · We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis, structural determination, …

Jan 17, 2023 · We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis, structural determination, …

Jul 26, 2022 · We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis, structural determination, …

Here, we report comprehensive investigation of ZnF456, leveraging X-ray crystallography and NMR to determine the structures in both the presence and absence of DNA. We delve into the dynamics and mode of interaction with DNA. Moreover, we discovered that the last zinc finger of BCL11A (ZnF6) plays.

We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis, structural determination, and molecular modeling to enhance binding affinity.

Jan 20, 2024 · BCL11A binds at a cognate recognition site (TGACCA) in the γ-globin gene promoter and represses its expression. DNA-binding is mediated by a triple zinc finger domain, designated ZnF456. Here, we report comprehensive investigation of ZnF456, leveraging X-ray crystallography and NMR to determine the structures in both the presence and absence ...

Dec 5, 2024 · We obtained the X-ray crystal structure of BCL11A ZnF456 (aa 730–835) bound to an 18-bp DNA oligonucleotide at 2.76 Å resolution, including for ZnF6. Coordinates were deposited in the PDB under accession number 8TLO (see Table S1 for structure statistics).

Oct 1, 2024 · We obtained first-generation nanobodies directed to a region of BCL11A comprising zinc fingers 4 to 6 (ZF456) from a synthetic yeast surface display library, and employed error-prone mutagenesis,...

Oct 17, 2024 · BCL11A binds to an optimal DNA promoter sequence TGACCA12,13 located at 118 to 113 in the g-globin locus. DNA binding is largely mediated by the C-terminus of BCL11A, comprising the last three of six zinc finger domains (ZnF456, amino acid [aa] 731–835 of BCL11A).14,15 Zinc fingers 4, 5, and 6 are each C 2H 2-type ‘‘treble-clef ...