ProbeLibrary, a fast, specific and flexible format for quantitative real-time PCR, is described. The ProbeLibrary concept is based on the fact that just 90 short probes provide transcriptome-wide ...
Using the procedure reported here, all the pipetting steps in LM-PCR can be performed on a Biomek 2000 liquid handling robotic workstation. After thermocycling is completed in the first reaction, the ...