Globin-chain synthesis in peripheral blood was studied by methods previously described. 13,14 In brief, peripheral blood was incubated with 10 μCi of L-leucine-U-14 C in a metabolic shaker at 37 ...

A boy with the β S /β S genotype, a single 3.7-kb α-globin gene deletion, and no glucose 6-phosphate dehydrogenase deficiency received a diagnosis of sickle cell disease at birth and was ...

Globin chains were quantified by densitometry using Alpha Imager 2000 software. In parallel, we also evaluated globin chains on a large-pore Vydac C 4 column 28.

Beta (β) globin chains. A normal adult haemoglobin molecule (called HbA) consists of two alpha and two beta chains. Thalassemia occurs when there is a mutation or deletion in the genes that code ...

Globin genes make the four globin chains from which the hemoglobin molecule is assembled. Humans have at least four globin genes — alpha, beta, gamma and delta.

Interestingly, people with beta-thalassemia major who also inherit an α globin gene deletion may have less severe symptoms. This is due to less of a mismatch between α and β globin chain ...

In HbA2, which makes up less than three percent of the total Hb protein, the alpha chains pair with a pair of delta globin chains. The delta globin gene sits upstream of the closely related beta ...

Alpha-globin and beta-globin protein chains make up hemoglobin. Changes in the alpha- or beta-globin genes that provide instructions for these proteins can lead to thalassemia.

The altered hemoglobin dampens polymerization of HbS and is also distinguishable from other globin chains using reverse-phase high-performance liquid chromatography.

The fetal hemoglobin counters the sickling of SCD or compensates for the deficiency of β globin chains in β thalassemia. However, the approach doesn't work for people who do not produce any β ...