Following this, 10 ng of cDNA was amplified by real-time qPCR in an AriaMx Real-time PCR System (Agilent Technologies, Santa Clara, CA, USA) using the TaqMan™ Universal Master Mix II kit (Cat. # ...

For RT-PCR analysis, we used the Single Cell Sequence Specific Amplification Kit (Vazyme, P621-01) and ChamQ Universal SYBR qPCR Master Mix (Vazyme, Q711-03) in accordance with the manufacturer’s ...

Trizol reagent used for RNA isolation, Reverse Transcription Kit used for reverse transcription of RNA to cDNA, and SYBR Green Master Mix used for qPCR, were obtained from Vazyme Biotech Co., Ltd.

The GoTaq ® Probe qPCR Master Mix includes dUTP. When dUTP is incorporated into the amplification products, the amplicons are susceptible to degradation by uracil-DNA glycosylase (UNG); allowing ...