We isolated mRNA from Blumeria graminis and prepared a cDNA library using a modification of Epicentre's ExactSTART ™ Full-Length cDNA Library Cloning kit. Analysis of the 5′ ends of the cDNA ...
consisting of about 18 600 fully verified cDNA clones, and a collection of full-length cDNAs (MGC collection) consisting of about 29 000 clones. The ORF library clones are available in Gateway-based ...
Repeat the dilution step and centrifuge again. Transfer the retentates containing cDNA to fresh 0.5-ml microcentrifuge tubes, and reduce the volumes to ∼10 μl in a rotary vacuum evaporator.
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