Southern, Northern, and Western Blot: What’s The Difference? The main difference between Southern, northern, and western blot techniques is the target molecules. While western blots target proteins in ...

This article describes the histone western blot protocol, which is often used at Abcam for identifying histone proteins obtained from purified calf thymus. For each lane, either 0.5 μg of calf thymus ...

Western blotting can often be more trouble than expected. While SDS-PAGE and western blotting protocols are straightforward, there are many critical decision points that lead to success or failure. In ...

Verify that the antibody is definitely able to detect the antigen in the species by checking data sheets and reference material Potential Cause: Primary and secondary antibody mismatch Raise the ...

Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Discover how decisions about experimental design can affect Western blotting reproducibility – and how to make the right decisions in this webinar from Sally Mulford, Sr Molecular Biologist at LI-COR ...

When it comes to Western blotting (WB), there is a wide range of different detection methods, reagents, and imaging systems ...

Gene tagging with fluorescent proteins is essential for investigations of the dynamic properties of cellular proteins. CRISPR–Cas9 technology is a powerful tool for inserting fluorescent markers into ...