Western blotting allows investigators to detect a specific protein in a complex mixture. Download this guide to discover tips on optimizing the gel and loading samples, antibody validation and ...

Western or immunoblotting analysis of protein expression in cells and tissues has been the major analytical tool for assessing molecular biological functions in basic cell biological research ...

She was using western blotting to study large ribosomal proteins, employing a protocol with two overnight steps. “Finally, I had that blot, and went to image it, but there was nothing there.