Western blot detection methods each have their own advantages and disadvantages. Choosing the right one can be a difficult decision. The basic protocol of the western blot – also referred to as the ...

Reviewed by Michael Greenwood, M.Sc. Fluorescent western blotting is a powerful protein detection technique that offers numerous benefits in comparison to alternative chemiluminescent or chromogenic ...

A western blot is a laboratory method used to detect specific protein molecules from among a mixture of proteins. This mixture can include all of the proteins associated with a particular tissue or ...

Aldrin V. Gomes, Ph.D. Ning Liu Ph.D. A closer examination of housekeeping and other normalization techniques. In recent years, the scientific community has questioned the reliability of Western ...

While Western blots appear straightforward, there is room for optimization at every step of the process to transform blurry blots into crisp, reliable results. In this Technique Talk sponsored by ...

Western blotting is one of the most ubiquitous procedures used in the life science research lab today. Commonly used to assess relative protein expression differences, accurate Western blot ...

Western bolting is a well-established method that is currently relied on in biology laboratories for determining the specific proteins and their abundance within a given sample of cells or tissue.

Dissecting cellular signalling requires the analysis of large number of proteins. The DigiWest approach we describe here transfers the western blot to a bead-based microarray platform. By combining ...

The western blotting technique that began as a means of detecting the protein of interest in a complex sample that may contain numerous other proteins has been in practice for four decades. In recent ...

For January’s Method of the Month I have selected the method presented by Kezzia S. Jones and colleagues in their recent reports article. The method utilizes binding to a surrogate protein (named ...

Mariella is an assistant editor at The Scientist. She has a background in neuroscience, and her work has appeared in Drug Discovery News and Massive Science. View full profile. Learn about our ...

Review this popular and powerful method for accurate detection of low abundance proteins or changes in protein expression, presented by Dr Martin Broadstock. Review the application of fluorescent ...