Figure 1: Separation of dodecylmaltoside-solubilized mitochondrial complexes exemplifying the native mass range (<100 kDa to ∼10 MDa) covered by BN-PAGE. Following solubilization of biological ...

Because of the relatively low cost of the ingredients, homemade acrylamide gels are the most economical choice. Scientists can generate numerous hand-cast gels for the same price as a single precast ...

Since the 18th-century researchers have studied proteins 1, but a number of discoveries in the 1960s that demonstrated sodium dodecyl sulfate (SDS) could facilitate the separation of proteins, ushered ...

In this article, we will consider how PAGE works, how it can be interpreted and some of its applications. What is polyacrylamide gel electrophoresis and what is protein electrophoresis? PAGE is a ...

Electrophoretic mobility shift assays (EMSAs) are commonly used to analyze nucleic acid–protein interactions. When nucleic acid is bound by protein, its mobility during gel electrophoresis is reduced.