A recent review highlights the critical role of DNA exonucleases and endonucleases in immune response and disease management, shedding new light on their potential applications in genomic stability, ...

This structural innovation pointed to the complex's ability to cleave DNA independently of the traditional components seen in similar CRISPR systems. By elucidating the role of the HNH nuclease ...

Next, the DNA is treated with a nuclease, such as DNAse I, that digests only unprotected DNA. Finally, the DNA products resulting from the digestion are separated on a polyacrylamide gel ...

In 2021, Karvelis discovered a compact RNA-guided protein capable of cutting DNA: TnpB. 3 Compared to other CRISPR nucleases ... 4.4-fold increase in the editing efficiency of this modified nuclease, ...

Recombinase efficiency increases allow Qi Biodesign to insert 20-30 kb (5 or 6 genes) at one time at one location across a variety of different crop genetics. In addition, a large team at Qi Biodesign ...

The amount of remaining host cell DNA was significantly reduced by nuclease treatment of the lentiviral supernatant. qPCR was used to quantify the DNA of residual host cells. Image Credit ...

To safeguard DNA and RNA from nonspecific cleavage by these molecular scissors, our cells support elegant mechanisms of regulation to ensure nucleases cut precisely. To uncover the mechanisms for ...

Researchers have revealed new details about the CRISPR-Cas5-HNH/Cascade complex, a variant of the type I-E CRISPR-Cas system, providing insights into its DNA recognition and cleavage mechanisms.