Full-length RecE and RecT from Rac prophage mediate highly efficient linear–linear homologous recombination that can be used to clone large DNA regions directly from genomic DNA into expression ...

Young scientists often get frustrated with laborious experimental procedures, such as cloning, PCR, or sequencing. However, the Internet has made planning and performing these procedures faster and ...

One of the most indispensable techniques in all areas of the life sciences is the use of expression vectors. Cloning a gene between two restriction sites, and the expression of a protein, are almost ...

Molecular cloning refers to the replication and recombination of DNA molecules. The first cloning experiments were carried out in the 1970s, when restriction endonucleases were discovered. Restriction ...

Molecular cloning is a technique used to create DNA experimental tools that are expressed in either bacterial or mammalian cells. Molecular cloning is defined as the isolation and amplification of a ...

This is a preview. Log in through your library . Abstract A clone bank of an indigenous plasmid of Enterobacter agglomerans containing structural nitrogen-fixation (nif) genes was established in a non ...

Whether it’s heat, emotions, or stress, the body sweats it out through a varied mix of volatile chemicals and microbes. A large language model can design and troubleshoot CRISPR protocols from scratch ...

The video above has some helpful tips for designing PCR primers for one purpose - cloning with restriction enzymes. PCR or Polymerase Chain Reaction, is a very common tool used in molecular biology to ...