Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into ...

SINGLE GUIDE RNA DESIGN AND PCR OPTIMIZATION We design and optimize CRISPR/Cas9 single or double guide RNAs for genome editing projects in mammalian cell lines. The service can be requested together ...

Advances in the gene-editing technology known as CRISPR-Cas9 over the past 15 years have yielded important new insights into the ...

Ryotaro Hashizume and colleagues used the CRISPR-Cas9 gene editing system to cleave the third chromosome in previously generated trisomy 21 cell lines derived from both pluripotent cells and skin ...

The second tool is the CRISPR-Cas9 system, which allows easy and precise editing of any region of the genome. When it comes to traditional immortalized cell lines, such as HeLa or HEK293, cutting with ...

But by combining a revolutionary new technology called CRISPR-Cas9 with a natural system known ... if CRISPR were used to edit a human embryo’s germ line—cells that contain genetic material ...

Scientists have found a way to study early embryonic development without real embryos. Using CRISPR, they programmed stem cells to self-organize into structures mimicking early embryos. The cells show ...

A recent review published in the journal Engineering delves into the significant advancements and potential of CRISPR ...

Using gene editing with CRISPR-Cas9 ... the interior of egg cells and embryos has been developed, resolving a major bottleneck to using the gene-editing tool CRISPR-Cas9 in many ...

European Patent Office grants new CRISPR/Cas9 patent (EP 4 289 948 ... targets for therapeutic intervention; development of cell lines for drug discovery and screening; bioproduction of antibodies ...

Plectin drives hepatocellular carcinoma progression and metastasis through cytoskeletal reorganization and oncogenic signaling, making it a promising therapeutic target for overcoming therapy ...

More information: Ryotaro Hashizume et al, Trisomic rescue via allele-specific multiple chromosome cleavage using CRISPR-Cas9 in trisomy 21 cells, PNAS Nexus (2025). DOI: 10.1093/pnasnexus/pgaf022 ...